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rabbit anti h3s10ph  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti h3s10ph
    A. Flow cytometry quantifying the fraction of cells past S phase by DAPI staining (left) and the fraction of cells actively in mitosis (right) by IF staining of <t>H3S10ph.</t> B. Enrichment profile plots of methyladenine at active transcription sites using H3s10ph targeted DiMeLo-cito in either mitotic (top) or unsynchronized GM24385 LCs (bottom) both smoothed with a 50 bp sliding window. C. Difference plot of profiles in B. D. Instances of CTCF M2 motifs identified by fimo in different centromere/satellite (censat) DNA annotated regions in the CHM13v2.0-T2T genome. E. Profile plots with 50bp smoothing of methyladenine at M2 motifs separated by censat annotations as well as all noncensat sites in the CHM13v2.0-T2T genome. F. Screenshot from IGV read browser at the H19 imprinting control region of anti-CTCF DiMeLo-cito in mitotic GM24385 LCs aligned to the HG002v1.1 genome.
    Rabbit Anti H3s10ph, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 261 article reviews
    rabbit anti h3s10ph - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis"

    Article Title: DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis

    Journal: bioRxiv

    doi: 10.1101/2025.03.11.642717

    A. Flow cytometry quantifying the fraction of cells past S phase by DAPI staining (left) and the fraction of cells actively in mitosis (right) by IF staining of H3S10ph. B. Enrichment profile plots of methyladenine at active transcription sites using H3s10ph targeted DiMeLo-cito in either mitotic (top) or unsynchronized GM24385 LCs (bottom) both smoothed with a 50 bp sliding window. C. Difference plot of profiles in B. D. Instances of CTCF M2 motifs identified by fimo in different centromere/satellite (censat) DNA annotated regions in the CHM13v2.0-T2T genome. E. Profile plots with 50bp smoothing of methyladenine at M2 motifs separated by censat annotations as well as all noncensat sites in the CHM13v2.0-T2T genome. F. Screenshot from IGV read browser at the H19 imprinting control region of anti-CTCF DiMeLo-cito in mitotic GM24385 LCs aligned to the HG002v1.1 genome.
    Figure Legend Snippet: A. Flow cytometry quantifying the fraction of cells past S phase by DAPI staining (left) and the fraction of cells actively in mitosis (right) by IF staining of H3S10ph. B. Enrichment profile plots of methyladenine at active transcription sites using H3s10ph targeted DiMeLo-cito in either mitotic (top) or unsynchronized GM24385 LCs (bottom) both smoothed with a 50 bp sliding window. C. Difference plot of profiles in B. D. Instances of CTCF M2 motifs identified by fimo in different centromere/satellite (censat) DNA annotated regions in the CHM13v2.0-T2T genome. E. Profile plots with 50bp smoothing of methyladenine at M2 motifs separated by censat annotations as well as all noncensat sites in the CHM13v2.0-T2T genome. F. Screenshot from IGV read browser at the H19 imprinting control region of anti-CTCF DiMeLo-cito in mitotic GM24385 LCs aligned to the HG002v1.1 genome.

    Techniques Used: Flow Cytometry, Staining, Control



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    Image Search Results


    A) Viability of Kc167 cells upon RNA interference (RNAi) with tip60 , dom-A , dom-B expression. Control cells were treated with RNAi against gst . Viability was measured using the CellTiter-Glo assay on day 4 and day 7 of RNAi treatment. Error bars: standard error of the mean (SEM) of 6 biological replicates for day 4 and 5 biological replicates for day 7. Different dsRNA sequences targeting the same transcript are indicated by #1 and #2. B) The data in (A) were replotted to reflect the duplication rate of the cells, as indicated. C) Mitotic index of cells (measured by H3S10ph staining) depleted of Tip60, Dom-A, Dom-B or control cells. Error bars: SEM of 4 biological replicates. D) The cell cycle profile of wild-type and control cells or cells lacking Tip60, Dom-A, Dom-B was determined by DAPI (4′,6-diamidino-2-phenylindole) staining followed by flow cytometry. E) Cell cycle phase distribution of the cell populations analyzed in (D). F) Quantification of S-phase in (D). Error bars: SEM of 5 biological replicates.

    Journal: bioRxiv

    Article Title: The Tip60 acetylome is a hallmark of the proliferative state in Drosophila

    doi: 10.1101/2025.07.15.664872

    Figure Lengend Snippet: A) Viability of Kc167 cells upon RNA interference (RNAi) with tip60 , dom-A , dom-B expression. Control cells were treated with RNAi against gst . Viability was measured using the CellTiter-Glo assay on day 4 and day 7 of RNAi treatment. Error bars: standard error of the mean (SEM) of 6 biological replicates for day 4 and 5 biological replicates for day 7. Different dsRNA sequences targeting the same transcript are indicated by #1 and #2. B) The data in (A) were replotted to reflect the duplication rate of the cells, as indicated. C) Mitotic index of cells (measured by H3S10ph staining) depleted of Tip60, Dom-A, Dom-B or control cells. Error bars: SEM of 4 biological replicates. D) The cell cycle profile of wild-type and control cells or cells lacking Tip60, Dom-A, Dom-B was determined by DAPI (4′,6-diamidino-2-phenylindole) staining followed by flow cytometry. E) Cell cycle phase distribution of the cell populations analyzed in (D). F) Quantification of S-phase in (D). Error bars: SEM of 5 biological replicates.

    Article Snippet: With the exception of the unstained and secondary antibody controls, the H3S10ph antibody (Active Motif, Cat. No. 39636, monoclonal antibody, mouse, clone MABI 0312) was added to the samples at a dilution of 1:500 and the samples were incubated on an overhead rotator for 2 hours at RT.

    Techniques: Expressing, Control, Glo Assay, Staining, Flow Cytometry

    A. Flow cytometry quantifying the fraction of cells past S phase by DAPI staining (left) and the fraction of cells actively in mitosis (right) by IF staining of H3S10ph. B. Enrichment profile plots of methyladenine at active transcription sites using H3s10ph targeted DiMeLo-cito in either mitotic (top) or unsynchronized GM24385 LCs (bottom) both smoothed with a 50 bp sliding window. C. Difference plot of profiles in B. D. Instances of CTCF M2 motifs identified by fimo in different centromere/satellite (censat) DNA annotated regions in the CHM13v2.0-T2T genome. E. Profile plots with 50bp smoothing of methyladenine at M2 motifs separated by censat annotations as well as all noncensat sites in the CHM13v2.0-T2T genome. F. Screenshot from IGV read browser at the H19 imprinting control region of anti-CTCF DiMeLo-cito in mitotic GM24385 LCs aligned to the HG002v1.1 genome.

    Journal: bioRxiv

    Article Title: DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis

    doi: 10.1101/2025.03.11.642717

    Figure Lengend Snippet: A. Flow cytometry quantifying the fraction of cells past S phase by DAPI staining (left) and the fraction of cells actively in mitosis (right) by IF staining of H3S10ph. B. Enrichment profile plots of methyladenine at active transcription sites using H3s10ph targeted DiMeLo-cito in either mitotic (top) or unsynchronized GM24385 LCs (bottom) both smoothed with a 50 bp sliding window. C. Difference plot of profiles in B. D. Instances of CTCF M2 motifs identified by fimo in different centromere/satellite (censat) DNA annotated regions in the CHM13v2.0-T2T genome. E. Profile plots with 50bp smoothing of methyladenine at M2 motifs separated by censat annotations as well as all noncensat sites in the CHM13v2.0-T2T genome. F. Screenshot from IGV read browser at the H19 imprinting control region of anti-CTCF DiMeLo-cito in mitotic GM24385 LCs aligned to the HG002v1.1 genome.

    Article Snippet: Antibodies used in this study were Rabbit anti-CTCF (Abcam) Mouse anti-CTCF (Thermo 1D11) Rabbit anti-H3s10ph (CST #53348) Rabbit Igg control(ab37415) and Mouse IgG Control (ab37355).

    Techniques: Flow Cytometry, Staining, Control